子痫前期（preeclampsia, PE）病因不明，胎盘因素为主要学说。我们前期研究和相关文献表明：miR-152与PE相关，可协助疾病预测；其靶基因人类白细胞抗原G（human leukocyte Antigen-G，HLA-G）可影响蜕膜 NK 细胞（decidual natural killer cells, dNK）功能。母胎界面中，dNK分泌相关因子，影响滋养细胞功能及血管重塑，维系正常妊娠。miR-152能否通过靶基因介导，参与dNK影响滋养细胞功能，且具有胎盘外泌体源性，值得探究。该项目拟构建转染miR-152的滋养细胞与dNK共培养体系，干预相关通路后检测dNK分泌功能，及共培养上清对滋养细胞侵袭、血管形成过程的影响；并对miR-152进行外泌体源性检测；探讨其能否通过对HLA-G的调控影响dNK细胞功能，进而参与PE发病的相关机制，及其是否具有成为PE疾病标志物的潜力。

The etiology of preeclampsia (PE) is not yet well-known, and the placental factor is the main etiology theory. Our previous studies and related literature showed that: miR-152 is associated with PE, and it could assist in disease prediction; its target gene human leukocyte antigen-G (HLA-G) could affect the function of decidual natural killer cells (dNKs). In the maternal-fetal interface, dNK affect trophoblast function and vascular remodeling by secretion of relevant cytokines, and maintain the normal pregnancy. It is worth to explore whether miR-152 could participate in the process of dNK affects the function of trophoblasts through be mediated by its target gene, and whether it has exosomes origin.On the basis of the previous study, this project intends to construct a co-culture system of trophoblast cells transfected with miR-152 and dNK, and to detect the secretion function of dNK after intervention of relevant pathways as well as the effect of co-culture supernatant on trophoblast invasion and angiogenesis; what’s more, to detect the exosomes origin of miR-152. To explore whether miR-152 could affect the function of dNK cells through regulation of HLA-G, and participate in the pathogenesis of PE; additionally, whether miR-152 has the potential to become a biomarker of PE.