乳腺癌是女性最常见恶性肿瘤，三阴性乳腺癌 (TNBC) 是乳腺癌特殊类型，化疗是TNBC主要治疗手段，TNBC耐药导致化疗失败，其分子机制复杂，解决TNBC化疗耐药是当前热点。肿瘤相关巨噬细胞（TAMs）在乳腺癌基质中大量存在并分泌细胞因子，诱导TAMs向M2型极化及P-gp蛋白在肿瘤细胞中过度表达是TNBC主要耐药机制；我们研究发现，参芪扶正注射液能降低TAMs释放炎症因子，抑制P-gp蛋白表达，提高TNBC化疗敏感性，其机制不明。.PI3k通路活性调控TAMs向M2型过度极化及P-gp表达。基于以上理论及我们研究基础，本课题拟通过体内与体外实验结合，分别增强或抑制PI3k通路活性，检测M2型TAMs比例及P-gp表达，观察耐药型TNBC对化疗敏感性，旨在证实参芪扶正注射液能通过调控PI3k信号通路活性，防止TAMs向M2型极化及抑制P-gp表达，改善TNBC耐药的增效机制。

Breast cancer is the most common malignant tumor in women. Triple negative breast cancer (TNBC) is a special type of breast cancer. Chemotherapy is the main treatment for TNBC. TNBC resistance leads to chemotherapy failure and its molecular mechanisms are complex. The solution of TNBC chemotherapy resistance is the current hot spot. Tumor associated macrophages (TAMs) are abundant in breast cancer matrix and secrete cytokines. Inducing the overpolarization of M2 and overexpression of P-gp in tumor cells are the main mechanisms of TNBC resistance. We found that ShenqiFuzheng injection reduced the release of inflammatory factors in TAMs, inhibited the expression of P-gp and improved the sensitivity of chemotherapy in TNBC. However, the mechanisms are unknown..The overpolarization of M2 and the expression of P-gp are regulated by PI3k. Based on this theory and our preliminary research, we enhance or inhibit the activity of PI3k pathway. Then the ratio of M2/M1 and P-gp expression are detected. The improvement of drug-resistant is observed in vitro and in vivo. These suggest that ShenqiFuzheng injection can prevent the polarization of M2, inhibit the expression of P-gp and improve drug-resistant in TNBC by regulating the activity of PI3k signaling pathway.