鼻咽癌是主要流行于中国南部和部分东南亚地区的恶性肿瘤。早期鼻咽癌并无特异性症状，患者出现症状而就医时大多已经进入中晚期，导致生存率明显下降。因此，鼻咽癌的早期诊断对于提高生存率、改善生活质量、降低治疗费用都有重要意义。研究表明，EB病毒（EBV）持续感染与鼻咽癌密切相关；血清中EBV抗体变化反映鼻咽癌的发生、发展过程，能用于早期筛查和诊断。本课题组曾利用基于VCA/IgA和EBNA1/IgA的风险评估方案进行鼻咽癌早期筛查，取得了很好的效果，但其假阳性率仍然很高。本课题拟通过全面表达EBV不同生活周期抗原而建立EBV抗体谱的化学发光检测平台，再通过鼻咽癌高发区癌前队列人群和各临床分期鼻咽癌患者的EBV抗体谱的动态检测，分析EBV抗体谱在鼻咽癌发生、发展过程中的变化特点，阐明鼻咽癌发生、发展过程中EBV不同生活周期蛋白的具体作用，从而为鼻咽癌的早期诊断和筛查提供新的更有参考价值的方案。

Nasopharyngeal carcinoma is a malignant tumor mainly prevalent in southern China and parts of southeast Asia.Patients with early nasopharyngeal carcinoma usually have no specific symptoms.However, advanced stages of tumor may have occurred when patients go for medical care，resulting in a decline in survival rate obviously.Therefore, it is of important significance for the early diagnosis of nasopharyngeal carcinoma (NPC) in improving the survival rate and improving the quality of life, as well as to reduce the cost of treatment.Studies have shown that EB virus persistent infection is closely related to nasopharyngeal carcinoma;EBV antibody detection in serum can reflect the occurrence and progress of nasopharyngeal carcinoma (NPC), which can also be used for early detection and screening.Our research group has conducted screening of early nasopharyngeal carcinoma (NPC) based on the VCA/IgA and EBNA1/IgA risk assessment scheme, which obtained the predictive effect,but the false positive rate was still high.This topic plans to establish chemiluminescence detection platform for EBV antibody spectrum based on the comprehensive expression of EBV antigen of different life cycles, and then detect dynamic changes of EBV spectrum of high incidence precancerous cohort of nasopharyngeal carcinoma (NPC) and NPC patients with different clinical stages and analyze the characteristics of EBV antibody spectrum changes during the occurrence and development process of nasopharyngeal carcinoma, clarifying the role of EBV specific antigens of different life cycles in oncogenesis and developmental progress, accordingly providing new reference value for the early diagnosis of nasopharyngeal carcinoma and screening.