经典Wg/Wnt信号通路与Hippo信号通路交叉对话调控果蝇发育的分子机制研究

批准号:
32000574
项目类别:
青年科学基金项目
资助金额:
24.0 万元
负责人:
伍洪刚
依托单位:
学科分类:
组织器官发育及体外构建
结题年份:
2023
批准年份:
2020
项目状态:
已结题
项目参与者:
伍洪刚
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中文摘要
经典Wg/Wnt通路和Hippo通路在进化中高度保守,参与调控细胞增殖、生长、分化和凋亡等基础生物学过程,通路的失调导致发育异常和肿瘤发生。器官的发育是多条信号通路协同调控的复杂过程。然而,对于器官发育过程中经典Wg/Wnt通路和Hippo通路相互作用的分子机制仍不清楚。用果蝇幼虫的翅成虫盘为研究模型,我们发现经典Wg/Wnt通路的活性可以负调控Hippo通路下游基因的转录,并且遗传学分析提示Wg/Wnt通路作用于Hippo核心激酶的上游。在前期研究基础上,本项目将用果蝇遗传学、分子生物学和生物化学方法,揭示经典Wg/Wnt通路和Hippo通路crosstalk的分子节点,阐明作用机制,并研究其在器官大小和个体发育中的生理功能。研究结果将有助于完善器官发育的信号调控网络,并为相关疾病的诊治提供理论依据。
英文摘要
The canonical Wg/Wnt pathway and Hippo pathway are evolutionarily conserved and both regulate fundamental biological processes, such as cell proliferation, growth, differentiation and apoptosis. Dysregulation of either pathway has been implicated in developmental abnormality and tumorigenesis. Organ development is a complex process coordinated by various signaling pathways. However, it remains unclear at the molecular level how Hippo and the canonical Wg/Wnt pathways interact, especially in the context of organ development. Using the Drosophila wing imaginal disc as a model, we found that the canonical Wg/Wnt pathway negatively modulates Hippo signaling output by genetically acting upstream of the core Hippo kinase cascade. Based on our preliminary results, the grant proposal aims to dissect the molecular mechanism underlying canonical Wg/Wnt and Hippo pathway crosstalk and elucidate its role in organ size control and animal development. The study will enhance our understanding of the signaling network in organ development and provide theoretical evidence for treatment of associated diseases.
期刊论文列表
专著列表
科研奖励列表
会议论文列表
专利列表
Shaggy regulates tissue growth through Hippo pathway in Drosophila
Shaggy 通过 Hippo 途径调节果蝇的组织生长
DOI:10.1007/s11427-022-2156-2
发表时间:2022-09-01
期刊:SCIENCE CHINA-LIFE SCIENCES
影响因子:9.1
作者:Wu,Honggang;Zhu,Nannan;Jiao,Renjie
通讯作者:Jiao,Renjie
DOI:10.1038/s41467-024-46455-w
发表时间:2024-03-06
期刊:NATURE COMMUNICATIONS
影响因子:16.6
作者:Li,Yue;Lu,Tianfeng;Huang,Hai
通讯作者:Huang,Hai
国内基金
海外基金
