磷是植物必需的矿质元素之一，而土壤吸附作用以及微生物同化作用经常造成植物可吸收的磷酸盐的缺乏，因此植物在进化过程中形成了适应低磷环境的多种策略。包括水稻在内的大部分陆生植物既可以通过根系的磷酸盐转运蛋白从土壤中直接吸收磷酸盐（直接吸收途径），也可以与菌根真菌共生，通过菌根真菌吸收磷（菌根共生途径）。本人博士期间在水稻中鉴定到一个GARP家族转录因子HINGE1，研究发现HINGE1可以激活参与直接吸收和菌根共生途径的磷酸盐转运蛋白基因的表达，同时其表达受菌根真菌侵染的显著诱导。本申请拟在已有研究基础上，首先对HINGE1遗传材料（过量表达转基因及突变体）的菌根真菌共生表型进行分析，并通过RNA-seq和ChIP-seq系统鉴定HINGE1的下游调控基因，筛选其互作蛋白，以HINGE1为切入点探究水稻协同调控菌根真菌共生增强磷吸收的分子机制，为提高水稻磷利用效率提供新思路及及新材料。

Phosphorus is one of the major mineral elements for plants. Due to the soil absorption and microorganism assimilation, the concentration of plant-accessible form, phosphate is often low in soil. Therefore, plants have evolved many strategies to adapt to the low phosphate environment. Many land plants including rice can absorb phosphate by direct root uptake and also through symbiosis with arbuscular mycorrhiza. Previously I identified a GARP family transcription factor, HINGE1, which can activate the expression of phosphate transporters involved in both direct Pi uptake and AM symbiotic Pi uptake. In addition, the expression of HINGE1 can be upregulated by AM symbiosis. To this end, we attempt to continue the study of HINGE1’s function in coordinated regulation of AM symbiosis for enhancing Pi uptake. We intend to characterize the AM symbiosis status in HINGE1 overexpression transgenic lines and hinge1 mutant, systemically identify the downstream targets of HINGE1 by RNA-seq and ChIP-seq, and screen for its interacting proteins. Through the above-mentioned studies, we hope to provide new ideas and breeding materials for improving phosphorus use efficiency of rice.