本项目拟以一种从白薇中分离而来的菲骈吲哚里西丁生物碱—安托芬（7-脱甲氧基娃儿滕碱）为对象，研究其对柑橘采后青霉病菌的抑制及作用机理。本研究通过扫描、透射电镜和激光扫描共聚焦显微镜观测安托芬对菌丝生长形态、超微结构、细胞膜完整性的影响；采用现代光谱如FDA-PI双色荧光、拉曼、傅立叶变换红外，及分子生物学技术研究安托芬对目标菌细胞膜结构、呼吸作用、营养物质、活性氧（ROS）和能量代谢的抑制作用，建立病原菌生长抑制动力学模型，探索其抑菌细胞生物学途径；通过基因芯片筛选出安托芬作用青霉菌前后的差异表达基因，在基因水平上解释药物的作用机制，并确定可能的作用靶点；同时借助RNA-Seq、TMT和GC-MS等组学技术阐明安托芬抑制柑橘采后青霉病发生的分子机理。研究成果为天然产物的抑菌机理研究提供切实可行的实验方法和理论依据，同时也为白薇的深度开发和综合利用鉴定理论基础，具有潜在的学术价值和社会效益。

Antofine (7-demethoxytylophorine) is a phenanthroindolizidine alkaloid isolated from the root of Cynanchum atratum and has significant antifungal activity against citrus postharvest blue mold. This project will be aimed to evaluate the possible antifungal targets and molecular mechanism of antofine against Penicillium italicum in order to control of citrus postharvest blue mold. The alterations of antofine on mycelial gmorphology, ultra-structure, cell membrane structures, respiration, and nutrients, ROS and energy metabolisms of P. italicum will be observed by the morphological interference assay of scanning electron microscope (SEM), transmission electron microscope (TEM), laser scanning confocal microscope (LSCM), FDA-PI, Raman, FT-IR, and molecular biotechnologies. The RNA-Seq, TMT, GC-MS and NMR analysis will be applied to provide the comprehensive analysis of the differential between antofine treated and control citrus fruit at three levels of gene, protein and metabolite. All of those will help us to explore the antifungal mechanism of antofine and explain the biological mechanisms of induced resistance control of blue mold in citrus fruit, caused by P. italicum. The research findings from this project will provide feasible methods and theoretical basis for screening and safety use of other natural product pesticides from plant origin in the control of postharvest fungal diseases in horticultural products. And it will also provide scientific basis for depth development and comprehensive utilization of Cynanchum atratum. So this project has important academic research value and application prospect.