PCOS颗粒细胞超生理分泌AMH对卵母细胞质量及发育潜能的影响

多囊卵巢综合征（PCOS）患者卵母细胞质量低下，常导致不受精、异常受精，由此形成低质量的胚胎导致不良的妊娠结局。抗苗勒氏管激素（AMH）在卵泡发育过程中发挥重要的作用，还可通过卵丘对话的形式调控卵母细胞的生长发育。我们的研究已证实，PCOS颗粒细胞中AMH转录启动子活性增强是导致卵泡液中AMH过度分泌的重要原因。在这种颗粒细胞本身存在异常的病理状态下，由其所致超生理分泌的AMH是否会抑制卵母细胞成熟及后续的胚胎发育？本课题是对前期研究的进一步深入，拟通过向大鼠卵丘复合物添加AMH重组蛋白及构建AMH过表达动物模型，模拟AMH异常分泌的PCOS卵泡微环境，探讨超生理水平分泌的AMH对卵母细胞质量及发育潜能的影响。本研究期望从卵泡微环境紊乱的角度揭示PCOS卵母细胞质量低下的机制，为今后改良PCOS临床治疗方案，抑制卵巢局部AMH过度分泌，改善卵母细胞质量探索新的途径。

Unfertilization and abnormal fertilization caused by poor quality of oocyte from polycystic ovary (PCOS) patients often results in unsatisfied pregnancy outcome. Anti-mullerian hormone (AMH) plays an important role in regulating follicular growth, also controls oocyte maturation in the way of oocyte-cumulus dialogue. It has been proved by our previous study that the enhanced activity of AMH transcriptional promoter in granulosa cells is the important cause of AMH excessive secretion in follicular fluid from PCOS patient. Will the supraphysiological production of AMH inhibit oocyte maturation and subsequent embryo development in the case that the granulosa cell itself has abnormal function? As the further research,the present study aims to mimic the micro environment in PCOS follicles and explore the effect of excessive secretion of AMH will on the oocyte quality and its developmental potential, by adding AMH recombinant protein into the culture medium of rat occyte-cumulus complexes (COCs) and establishing AMH over-expressing animal model. We hope to reveal the pathological mechanism in which the oocyte quality is undermined, thus it helps to modify PCOS therapeutic protocol, depress the over-production of AMH in ovary, and improve oocyte quality.