重复经颅磁刺激（rTMS）可提高阿尔兹海默病（AD）患者的认知功能。L型电压依赖性钙通道（L-VDCC）在海马神经元主要存在两种亚型：CaV1.2和CaV1.3。前期研究证实，应用高频rTMS可以通过抑制VDCC，降低神经元后超极化幅度，上调神经元兴奋性，改善增龄引起的认知功能障碍。本课题拟在我们上述工作的基础上，应用新物体识别实验、免疫组化技术、Western blot技术、mRNA干扰技术、寡核苷酸技术、膜片钳全细胞记录技术，观察CaV1.2和CaV1.3在rTMS改善AD小鼠认知功能过程中的表达及神经元在rTMS后兴奋性的变化，抑制和增加CaV1.2和CaV1.3表达及其功能对rTMS调节神经元兴奋性作用的影响，探讨CaV1.2和CaV1.3在rTMS改善AD小鼠认知功能过程中发挥作用的具体机制，为AD的治疗提供新思路。

Repetitive transcranial magnetic stimulation (rTMS) could improve the cognition of individuals with Alzheimer's disease (AD). L-type voltage-dependent calcium channels (L-VDCC) is the main channel mediated hyperpolarization of the neurons in the hippocampal neurons, and there are two major subtypes: CaV1.2 and CaV1.3. Our previous study confirmed that application of high frequency rTMS improved age-related cognitive dysfunction by reducing neuronal hyperpolarization amplitude and increased neuronal excitability. This study intends to show what L-VDCC subtypes play a critical role in rTMS to improve cognitive function in AD mice. After the application of objective recognition test, immunohistochemistry, Western blot, mRNA interference, oligonucleotides, whole-cell patch clamp recording technique, this study intends to observe the expression of CaV1.2 and CaV1.3 after the improved cognition in AD mice and the neuronal excitability changes by rTMS. After Inhibition the functional of CaV1.2 and CaV1.3, in this study we plan to observe the regulation of neural excitability effects of rTMS, to explore CaV1.2 and CaV1.3 specific mechanisms to improve cognitive function in AD mice in the process of rTMS application, providing a new way for the treatment of AD.