内脏高敏感性是肠易激综合征（IBS）重要发病机制之一，TRPV1敏化是介导内脏高敏感发生的关键位点，肥大细胞（MC）活化是内脏高敏感发生的始动环节，其调控机制与Tryptase-PAR2-PKCε通路密切相关。前期临床研究证实腹部推拿治疗IBS内脏痛疗效肯定，基础研究表明腹部推拿可以有效干预IBS中枢敏化，但对肠道局部外周敏化的机制缺乏深入探究。本项目是既往国自然课题延伸，以IBS内脏痛为突破口，基于Tryptase-PAR2-PKCε通路敏化TRPV1，从靶点多环节、检测多水平上探讨腹部推拿治疗IBS机制，以期证实“腹部推拿通过机械力信号向内传输，作用肠道MC，调控MC脱颗粒，控制Tryptase释放，并借助Tryptase-PAR2-PKCε通路，调控TRPV1敏化，进而改善肠道高敏感性”假说的真实性，进一步揭示腹部推拿干预IBS的作用机制，为构建腹部推拿治疗消化系统疾病提供科学依据。

Visceral hypersensitivity is one of the important pathogenesis of irritable bowel syndrome (IBS). Sensitization of TRPV1 is a key site to mediate the development of visceral hypersensitivity, and the activation of mast cells (MC) is the starting point of visceral hypersensitivity. Its regulatory mechanism is closely related to the Tryptase-PAR2-PKCε pathway. Preclinical studies have confirmed the efficacy of abdominal Tuina in the treatment of IBS visceral pain. Basic research shows that abdominal Tuina can effectively interfere with the central sensitization of IBS, but the mechanism of local peripheral sensitization of the intestine lacks in-depth exploration. This project is an extension of the previous natural issues in the country, using IBS as a breakthrough in visceral pain, sensitizing TRPV1 based on the Tryptase-PAR2-PKCε pathway, and exploring the IBS mechanism of abdominal Tuina with multiple levels of detection and multiple levels of detection, in order to confirm that “abdominal Tuina The mechanical force signal is transmitted inward, acts on the intestinal MC, regulates MC degranulation, controls Tryptase release, and utilizes the Tryptase-PAR2-PKCε pathway to regulate the TRPV1 sensitization, thereby improving the authenticity of the intestinal hypersensitivity hypothesis, further revealing Abdominal Tuina intervention IBS mechanism of action, to provide a scientific basis for the construction of abdominal Tuina treatment of digestive diseases.