现有的临床检测手段难以对甲状腺微小乳头状癌(PTMC)的生物学特性进行准确评估。本课题前期研究发现，WNT10A基因在PTMC癌组织中高表达，并与侵袭、转移相关，WNT10A基因敲减能显著抑制PTMC癌细胞的增殖及侵袭、转移，测序结果表明WNT10A通过Tec信号通路激活STAT3，抑制Tec/STAT3能够抑制PTMC癌细胞和肿瘤干细胞的存活。据此我们推测WNT10A为潜在的PTMC分子诊断标记物，其通过Tec/STAT3信号通路促进PTMC癌细胞和肿瘤干细胞的增殖、侵袭与转移。本项目拟在细胞和动物模型中深入探讨WNT10A在PTMC侵袭、转移中的作用和分子机制，并在此基础上结合超声特征建立可以准确预测PTMC侵袭性的风险预测模型，为PTMC患者诊断及选择制定合理的临床治疗方案提供重要的指导意义。

The current clinical testing method is difficult to accurately evaluate the biological characteristics of thyroid papillary carcinoma (PTMC). Our previous study found that WNT10A gene was highly expressed in PTMC tissues and was associated with invasion and metastasis. Knockdown of WNT10A could significantly inhibit the proliferation, invasion and metastasis of PTMC cancer cells. RNA-seq showed that WNT10A activates STAT3 through Tec signaling pathway. Inhibition of Tec/STAT3 prevents the survival of PTMC cancer cells and cancer stem cells. We speculated that WNT10A is a potential molecular marker for PTMC diagnosis and promotes the invasion and metastasis of PTMC through Tec/STAT3 pathway. This project intends to investigate the role and molecular mechanism of WNT10A in promoting invasion and metastasis of PTMC, establish a risk prediction model for predicting PTMC invasiveness.This project would provide important guidance for the diagnosis and selection of reasonable clinical treatment plan for PTMC patients.