槲蕨（Drynaria roosii）是我国传统中药材，是骨碎补商品的主要来源，由于需求量大而人工栽培技术相对落后，导致野生槲蕨资源日益枯竭。因此，迫切需要建立槲蕨的人工保存和快繁体系。蕨类植物的绿色球状体（Green Globular Bodies，GGBs）具有继代培养容易、繁殖倍率高且周期短的特点，已越来越多的用于蕨类种质的离体保存和人工快繁中。本项目围绕槲蕨离体保存和人工繁殖中的关键问题，即槲蕨GGBs的形态特征和生长发育规律，进行以下研究：(1) GGBs诱导和分化过程中的关键结构特征；(2) GGBs增殖组织和分化组织的空间分布和位置关系；(3) GGBs胚性细胞的结构特征和胚性组织的分布模式，旨在揭示槲蕨GGBs的形态学本质及其形态发生机制。研究结果对深入阐明蕨类植物的发生发育机制具有重要的科学意义，为建立骨碎补原料植物槲蕨的离体保存和人工繁殖技术提供支撑。

Due to the huge demand for Drynaria roosii, the main source of the Chinese traditional medicine “Gu-Sui-Bu”, the D. roosii wild resources is becoming increasingly over-exploited. Therefore, it is urgently needed to establish artificial conservation and breeding technology of D. roosii. Green Globular Bodies (GGBs), induced by 6-BA and/or NAA during fern tissue culture, are excellent for in vitro conservation and artificial propagation of ferns, owing to high multiplication rate and reproduction efficiency of GGBs. Here, we focus on some key questions regarding to the structural character and developmental law of D. roosii GGBs during in vitro conservation and artificial propagation: (1) morphological and structural features of GGBs during induction, multiplication and differentiation, (2) distributional difference between the proliferative cells and the differentiated ones, (3) ultrastructure of embryonic cells and their distribution pattern in GGBs. The results will contribute to revealing the morphological essence and the morphogenetic mechanism of GGBs in D. roosii. The present study may provide deeper insights into the morphogenetic and developmental mechanism of ferns, thereby playing important roles in establishing the in vitro conservation and artificial propagation technology system of D. roosii.