多溴二苯醚(polybrominated diphenyl ethers, PBDEs)是一种非常优质的阻燃剂，在日常生活中有广泛的应用，但是PBDEs及其代谢产物对甲状腺系统的机能具有很强的搅乱作用，尤其是对儿童的危害更为严重,已被研究人员所关注。课题使用普通斑马鱼胚胎以及3型脱碘酶-荧光转基因斑马鱼胚胎作为实验动物模型，研究我们在日常环境中所接触的PBDEs，检测PBDEs（以6-OH-BDE-47为代表）所引起的斑马鱼显著的形态学变化（如胚胎旋转运动的增加、色素沉积的降低等），评估甲状腺素受体（THRβ）表达的降低、3型脱碘酶等基因的表达的升高等变化，使用基因敲除、过表达等技术确认其特异性，定性、定量地高通量检测PBDEs的环境毒性。同时使用化学方法评价PBDEs在斑马鱼体内的分解代谢，建立完整的生物学评价体系。

Polybrominated diphenyl ethers (PBDEs) and their metabolites (hydroxylated PBDEs) are excellent flame retardants and are widely used today. However, it is reported that most of these chemicals are endocrine disruptors and have been shown to disrupt thyroid hormone system regulation, especially in fetuses and children. We will use zebrafish embryos and Deiodinase3-GFP transgenic embryos as an animal model to investigate the toxicity of PBDEs on morphological changes (i.e. increased circle movement and decreased pigmentation), decreased thyroid hormone receptorβ (THRβ) mRNA expression, and increased deiodinase 3 etc. We will carry out qualitative and quantitative detection for the toxicity of PBDEs using gene knock down (out) or mRNA over expression to identify the specificity of the effects, and concurrently use chemical analysis method to evaluate the metabolism of thyroid hormones in these zebrafish. The purpose of this study is to establish good biomarkers to assess the risk of PBDEs in the environment.