独特的化学及药理学多样性使得蜘蛛毒液在发展新型药物和杀虫剂导分子以及神经生物学工具试剂等方面发挥着重要作用。本研究组利用LC-MALDI方法在大腹园蛛毒液中发现了一种新型的非肽类小分子毒素AVTX-623，这种毒素能特异性作用于昆虫神经细胞，而对哺乳动物无明显毒性。本研究拟对此该毒素进行研究以发现特异性作用于昆虫神经细胞的分子机理。综合运用色谱技术、膜片钳技术、红外光谱、生物质谱技术及核磁共振等技术揭露该非肽类小分子毒素的结构与功能。期望能揭露其对昆虫的作用机制，为新型生态杀虫剂先导分子及杀虫靶标的发现提供新的线索。

With the extraordinary chemical and pharmacological diversity, spider venom has been playing important role for developing novel insecticidal lead and neurobiological tool reagent. A kind of nonprotein low molecolar weight component (AVTX-623) was firstly found in the venom of Chinese spider Araneus ventricosus using LC-MAlDI-TOF. The primary result indicated that the component target selectively against insects, it is ineffective in vertebrates.Hence the study makes a plan for discovering the mechanism of selectively toxicity of the toxin against insects. A combined chromatogram，patch clamp, infrared spectroscopy, biological mass spectrometry and NMR approach was employed to identify structure and function of the toxin. And the study was expected to found the action mechanism of the toxin against insect, which will give novel clue for developing novel pesticidal lead and target.