腰椎间盘突出症(LDH)发病率高及年轻化趋势增加了世界医疗消耗，业已成为严重的社会问题。基因多态性的研究是目前国际研究热点，聚集蛋白聚糖(Aggrecan)是椎间盘基质中的重要成分。国外报道显示Aggrecan基因串联重复多态性(VNTR)与肥胖分别参与LDH的发病。我们的前期研究首次报道Aggrecan VNTR与肥胖存在交互作用，协同影响LDH发病，然而其具体机制尚不明确，前期研究成果发表于Spine、中华外科杂志等期刊。本课题旨在应用较大样本深入研究Aggrecan VNTR与瘦素/脂联素交互作用对LDH易感性的影响机制。本研究有望为认识和防治LDH提供新线索，并进一步建立LDH易感人群筛查识别系统，对该病的早期筛查及精准诊疗具有重要意义。

Symptomatic lumbar disc herniation (LDH) is a familiar orthopaedics disease, and its incidence increased year by year. The majority of patients with Symptomatic LDH presented with leg and back pain. Despite numerous studies of its etiology and pathogenesis, it is not clear why susceptivity of symptomatic LDH is low in some individuals while high in others. Gene polymorphism has become the new research subject in the orthopaedics field of the world. There have been reported that Aggrecan Variable number of tandem repeats polymorphism (VNTR) and obesity correlated with lumbar disc herniation respectively. We found the interaction between aggrecan gene VNTR polymorphism and obesity in predicting incident symptomatic lumbar disc herniation. However, the mechanism of the interaction between the aggrecan VNTR and obesity with susceptibility of symptomatic lumbar intervertebral disc degeneration disease has not been well studied. Therefore, the development of this study has the very vital significance. This study aims to screen, detect and verify the distribution of aggrecan gene polymorphism in a Large sample, through the determination of the content of aggrecan，chondroitin sulfate (CS), the profile of aggrecan fragmentation, aggrecanase, MMPs, leptin, adiponectin in the samples with the same repeats of Aggrecan gene VNTR, to ascertain the mechanism of the association between the aggrecan gene VNTR and obesity with susceptibility of symptomatic LDH.