腰椎间盘突出症（LDH）是骨科常见病之一，发病率逐年增高。基因多态性的研究是继人类基因组计划之后又一国际研究新热点。有报道称：在人类退行性变间盘组织发现了大量的Aggrecanase和Aggrecan酶解碎片。然而关于Aggrecan和Aggrecanase的基因多态性与腰椎间盘突出症易感性之间的相互关系还没有被很好的研究。因此本课题的开展具有十分重要的意义。本课题通过相对较大样本进一步筛查、检测、验证病例组和对照组中Aggrecan和ADAMTS4/5基因多态性；通过进一步测定相同Aggrecan基因串联重复序列样本中，ADAMTS4/5的酶活性、Aggrecan大分子和裂解片段含量、硫酸软骨素含量、ADAMTS4/5在间盘组织中的表达量，来研究Aggrecan和Aggrecanase的基因多态性对腰椎间盘突出症易感性的影响及其机制，对临床腰椎间盘突出症的防治开拓了有价值的新思路。

Symptomatic Lumbar disc Herniation (LDH) is a familiar orthopaedics disease, and its incidence increased year by year. The majority of patients with symptomatic lumbar disc herniation (LDH) presented with leg and back pain. Despite numerous studies of its etiology and pathogenesis, it is not clear why susceptivity of symptomatic LDH is low in some individuals while high in others. Gene polymorphism has become the new research subject in the orthopaedics field of the world. Aggrecanases and aggrecanase-generated aggrecan fragments have been found in human degenerated discs. However, the mechanism of the association between the gene polymorphism of aggrecan and aggrecanase(ADAMTS-4, ADAMTS-5) with susceptibility of symptomatic lumbar disc herniation has not been well studied. Therefore, the development of this study has the very vital significance. This study aims to screen, detect and verify the distribution of aggrecan and ADAMTS4/5 gene polymorphism in a Large sample, through the determination of ADAMTS4/5 enzyme activity, the content of aggrecan, chondroitin sulfate (CS), ADAMTS4/5 and the profile of aggrecan fragmentation in the samples with the same repeats of Aggrecan gene VNTR, to ascertain the mechanism of the association between the gene polymorphism of aggrecan and aggrecanase(ADAMTS-4, ADAMTS-5) with susceptibility of symptomatic lumbar disc herniation.