拟南芥C3H14介导依赖和不依赖WRKY33途径抵御灰霉病的分子机理研究

灰霉病是一种严重危害农业生产的真菌病害，植物在抵御灰霉病过程中形成了完善的调控机制，但目前植物防御灰霉病的分子机理仍不清晰。我们前期研究表明，拟南芥CCCH锌指蛋白C3H14不仅能依赖抗灰霉病核心调控因子WRKY33来激活茉莉酸/乙烯信号和促进植保素合成，而且也能不依赖WRKY33直接激活ORA59的表达、提高活性氧清除能力和调控细胞壁形成来抵御灰霉病。本项目以此为切入点，利用RNA-seq和ChIP-qPCR等技术全面鉴定C3H14抗病相关靶基因；利用遗传杂交和ChIP-qPCR等技术明确C3H14与WRKY33的关系，系统比较C3H14信号和WRKY33信号调控灰霉病分子网络差异；利用HPLC等技术明确C3H14调控细胞壁形成和抵御灰葡萄孢菌侵染的内在联系，最终解析C3H14参与调控灰霉病的分子机制。研究结果将为抗灰霉病作物新种质的创制奠定理论基础。

Botrytis cinerea is a major plant fungal pathogen, causing serious harm to agricultural production. However the molecular mechanisms of plants defend B. cinerea infection remain unclear. Our previous study has showed that Arabidopsis CCCH zinc finger protein C3H14 activate jasmonic acid/ethylene signaling and increases the content of phytoalexins in WRKY33-dependent manner to against B. cinerea infection, and active the expression of ORA59, enhance oxygen scavenging ability and regulate cell wall formation upon B. cinerea infection in WRKY33 -independent manner. Based on these results, this project will identify disease-related targets of C3H14 using RNA-seq and ChIP-qPCR technologies; determine the genetic relationship of C3H14 and WRKY33 using crossing and ChIP-qPCR technologies; compare C3H14- and WRKY33-mediated signalling networks during B. cinerea infection; explore the relationship of C3H14 controlling cell walls and the B. cinerea resistance using HPLC technology, and finally clarify the regulatory mechanism of C3H14 in the B. cinerea defense. The results in this project may provide a theoretical basis for the cultivation of a new crop germplasm with the B. cinerea resistance.