作物野生近缘种往往蕴含着抗病、抗逆、高产等性状。疣粒野生稻对水稻白叶枯病近乎免疫，但由于其染色体组型不同，无法与栽培稻进行有性杂交，其抗性基因尚未被有效鉴定利用。项目组在前期研究中，通过体细胞杂交和基因定位等工作，获得了多个疣粒野生稻抗白叶枯病候选基因。通过基因克隆及转基因验证，发现其中一基因（OmBBR1）对白叶枯病具有显著抗性。本项目拟在此基础上，对这一来源于疣粒野生稻的抗病基因进行深入的抗病机理研究。首先，对现有转基因材料进行纯合鉴定、扩繁及抗谱分析，同时对该基因的结构、表达等特性进行分析。然后利用基因芯片、荧光蛋白双向电泳等方法分析OmBBR1如何调控水稻抗病途径。最后，尝试采用酵母双杂交等方法分析其潜在的互作基因，并用双分子荧光互补等手段进行验证。通过此项目的实施，将使我们进一步了解疣粒野生稻抗病基因在水稻抗白叶枯病防御反应中的作用，同时为更多、更好地利用野生稻种质资源提供线索。

Closely related species of crops often contain many agronomic valuable traits, such as disease resistance, stress tolerance, and high yield. Oriza.meyeriana is almost immune to rice bacterial blight. However, because of the sexual incompatibility, its bacterial blight resistance has not been extensively utilized. In previous studies, we introduced the bacterial blight resistance trait from O. meyeriana into O. sativa using asymmetric somatic hybridization with O. meyeriana as the donor species, and successfully identified several candidate genes. In transgenic analysis, one gene (OmBBR1) exhibited high bacterial blight resistance. This study focuses on the functional characterization of this disease resistacne gene. First, we will identify the homozygous transgenic lines, prepare more plants for resistance spectrum test, and analyze the structure, expression pattern and other characteristics of OmBBR1. Then the transcriptome and 2-D protein electrophoresis analysis will be applied to identify the defence pathways that OmBBR1 involved in. Finally, the yeast two-hybrid or other methods will be used to find the potential interactors of OmBBR1, and the BiFC or other assay will be carried out to verify the interactions. This research will help us further understand the role of O.meyeriana resistance genes in rice bacterial blight defense reaction, and provide clues for utilizing the gene resources in wild rice.