游离脂肪是最理想的软组织填充剂之一，移植后的脂肪再生被认为是决定其转归的关键。课题组前期研究发现：游离脂肪移植后，部分成熟脂肪细胞出现脂滴分解伴棕色化的转变。雌激素受体（ER）选择性激动剂他莫昔芬被发现可特异性诱导白色脂肪棕色化并显著提高移植脂肪的保留率，但具体机制尚不明确。本研究拟采用组织移植技术、细胞生物学技术、蛋白生化技术、病理检测等技术，观察他莫昔芬选择性激活ERα后ERα-Nrf1/2信号通道与胞内线粒体生成的关联，探索该通路对游离脂肪移植后脂肪再生的影响。本研究将进一步揭示雌激素受体通道在调控白色脂肪棕色化过程中的重要作用，探讨白色脂肪棕色化预处理后提高游离脂肪移植后脂肪再生的潜在价值及可能机制。

Autologous fat tissue is one of the most ideal soft tissue fillers. Adipose tissue regeneration after transplantation is the key to determining fat grafting outcome. Our preliminary study found that mature adipocytes could undergo a process of oil droplet decomposition showing features of adipocyte browning after free fat transplantation. The estrogen receptor (ER) selective agonist tamoxifen was found to specifically induce browning of white fat and significantly increase the retention rate of transplanted fat, but the specific mechanism is not clear. This study intends to use tissue transplantation technology, cell biology technology, protein biochemical technology, pathological detection and other techniques to observe the association between ERα-Nrf1/2 signaling pathway and intracellular mitochondrial production after tamoxifen selectively activates ERα. Its effect on fat regeneration after free fat transplantation will also be evaluated. This study will further reveal the important role of estrogen receptor and its related pathway in the regulation of white fat browning, and explore the potential value and possible role of browning pretreatment on fat regeneration after free fat transplantation.