自杀质粒对再生水中病原微生物的抑制机理研究

再生水中病原微生物引起的健康风险，是我国再生水储存回用战略可持续性发展的一个重要影响因素。在再生水回用过程中，病原微生物常以低活性状态存在，导致病原微生物种群分布不均匀和感染致病剂量不稳定，进而掩盖了其侵袭活性并增加了其再爆发风险，其风险防控已成再生水治理的关键。本研究以再生水中隐孢子虫和肺炎克雷伯氏菌为研究对象，从利用自杀质粒抑制水体中病原微生物为切入，拟开展以下研究：（1）再生水中病原微生物种群沉默、再爆发机理分析；（2）建立再生水储存回用体系中病原微生物爆发风险预测模型；（3）自杀质粒系统（致死基因元件和启动子控制元件）的编辑和构建；（4）自杀质粒感染再生水中目标病原微生物种群效率评估。预期成果不仅能为再生水储存回用中病原微生物的风险防范与控制提供理论基础与技术支撑，还能为污水再生领域的发展做出相应的贡献，有较强的理论与实用价值。

The health risk of pathogenic microorganisms in reclaimed water influences its sustainable development. In the process of reclaimed water recharging and reuse, pathogenic microorganisms with low activity state exist in the water, which leads to uneven distribution of population of pathogenic microorganisms and instable pathogenic dose, obscure invasive activity and increasing risk of re-exposure. This research aims to utilize suicide plasmids to suppress Cryptosporidium and Klebsiella pneumoniae  in reclaimed water. The objectives of this research include: (1) analysis of health risk and population structure of pathogenic microorganisms in reclaimed water; (2) study on mechanisms of   suicide plasmids controlling population of pathogenic microorganisms; (3) application of suicide plasmids suppressing pathogenic microorganisms in reclaimed water; (4)the efficiency and response mechanism of suicide plasmid infection 0n target microorganisms in reclaimed water.The research results can not only provide the theoretical basis and technical support for reclaimed water storage and pathogenic risk prevention and control, but also make contribution for the development in the corresponding field of sewage regeneration. There are strong theoretical and practical values in this project.