胶原XV（Collagen XV）是脂肪细胞外基质的重要组分，并与白色脂肪细胞分化和脂质代谢密切相关。本课题组已发现猪体内存在白脂棕色化现象，且在冷刺激的仔猪皮下和内脏白脂中ColXV表达显著提高，提示其可能参与白脂棕色化作用，但具体作用及机制尚不清楚。本项目拟以关中黑猪为试验对象，通过实时定量PCR、蛋白杂交、荧光探针、激光共聚焦显微镜、膜片钳、基因芯片等技术，探索猪白脂棕色化中ColXV表达规律及Ca2+浓度变化，研究ColXV对猪白脂棕色化的调节作用并筛选关键基因及相关信号通路，探讨Ca2+信号对ColXV调节猪白脂棕色化的影响，揭示“Ca2+-内质网”轴在ColXV调节猪白脂棕色化中的作用，探明ColXV转录因子及其对猪白脂棕色化的调节作用；为深入研究猪白脂棕色化的遗传调控、降低猪脂肪沉积、提高瘦肉率提供理论依据，为防治人类脂代谢紊乱、肥胖症等疾病提供新思路。

Collagen XV is the important components of the extracellular matrix in adipocytes, which have been confirmed closely related to white adipocytes differentiation and lipid metabolism. Our group further finds that the browning of the white adipose exists in pig. And the ColXV expression can be significantly induced by cold stimulation in piglet subcutaneous and visceral white adipose, indicating ColXV may take part in the regulation of white adipose browning, but the molecular mechanism is still unclear. In this study, Guanzhong black pigs are used as the experimental animals. Through qRT-PCR, Western Blotting, fluorescence probe, laser scanning confocal, patch clamp gene chip technology and other technics are applied. We investigate the expression pattern of ColXV during white adipocyte browning in pig, as well as the change of intracellular Ca2+ concentration, resolve the regulating effect of ColXV on white adipocytes browning in pig and filtrating the key genes, the significant signaling pathway, explore the relationship between Ca2+signal and the regulation of ColXV on browning, reveal the important role of "Ca2+-ER" axis on the regulation of ColXV on adipocyte browning in pig, and explain the transcription regulation of ColXV and its effect on adipocyte browning in pig. Results would lay a theoretical basis for further study of the genetic manipulation of white adipose browning in pig，the reduction of fat deposition in pigs and the increase of lean meat percentage. It also could offer a new strategy for the prevention and treatment of human dyslipidemia, obesity and related diseases.