Dnmt3基因为调控蜜蜂级型分化的关键基因，在雌性蜜蜂幼虫期，它通过改变下游基因的DNA甲基化状态来调控雌性蜜蜂的级型分化。我们的前期研究表明 Dnmt3基因在蜂王幼虫和工蜂幼虫分化的关键时期存在mRNA转录差异，但是对于造成这种转录差异的调控机理还不清楚。本研究期望鉴定出蜜蜂（Apis mellifera ligustica）Dnmt3基因启动子的关键转录调控区域，鉴定出结合在这些关键调控区域的转录因子，对这些转录因子进行功能分析。希望通过这些研究弄清蜜蜂Dnmt3基因受到转录调控的具体分子机理，完善蜜蜂级型分化调控网络。

Dnmt3 is a key gene for honeybee caste differentiation. It regulates the caste differentiation of female honeybee by altering the DNA methylation state of its downstream target genes at the larvae stage. Our previous studies have found that the honeybee Dnmt3 gene exhibit differences in mRNA transcription between queen larvae and worker larvae during the critical period of larvae caste differentiation. However, the mechanism leading to this difference is unclear. This study was aimed to identify the critical transcriptional regulation regions of the honeybee (Apis mellifera ligustica) Dnmt3 promoter, identify transcription factors binding to the Dnmt3 promoter, and analyze the function of the identified transcription factors. Through these studies, we hope to clarify the regulation mechanism of honeybee Dnmt3 gene from the molecular level, and make the regulatory network of the honeybee caste differentiation become completed.