我国不孕症发病率已达10%以上。人类辅助生殖技术（试管婴儿）是目前解决不孕症最为有效的治疗方法，但胚胎种植率偏低始终是生殖医学领域尚未解决的问题之一，也是限制人类辅助生殖技术发展的瓶颈。子宫内膜容受性是影响胚胎种植率的最关键因素，近年来相关研究取得了一些成果，但目前仍未找到能够快速、方便、准确判断内膜种植窗口期的有效方法。值得注意的是，相关研究大多集中于内膜组织的转录组水平，针对内膜移植窗口期的血浆差异蛋白的研究，国内外鲜有报道。.本项目拟利用DIGE-2D联合MALDI-TOF-MS技术对接受IVF-ET治疗的女性患者移植当天的血浆进行分析，结合内膜组织转录组学水平的研究结果，以期寻找到能够准确预测子宫内膜种植窗口期的生物标志物，同时应用其指导临床胚胎移植。其研究结果对提高我国试管婴儿成功率具有十分重要的科学意义，同时对千万个翘首以盼宝宝的家庭来说具有更加重大的社会意义。

Human Assisted Reproductive Technology is the most effective treatment for infertility patients whose prevalence has been over 10% in China, but the low rate of embryo implantation has always been one of the outstanding problems to solve. Recent studies on endometrial receptivity which is the most critical factor that may relate to embryo implantation rate have made some achievements. However, there is still a serious lack of convenient and effective ways to identify the window of implantation. Moreover, most of these studies were focus on the transcriptomic level of endometrial tissue but proteomics research is rarely reported.. Therefore, we plan to explore protein biomarkers in the plasma of IVF-ET patients by two-dimensional electrophoresis combined with the matrix-assistedlaser desorption-ionization time-of-flight mass spectrometry. This proteomic analysis study will be for the first time carried out in the plasma of IVF-ET patients to identify the window of implantation. These will be very important for scientific significance to improve the successful rate of IVF, but also a more important social significance for millions of infertility families.