海葵蛰人后释放的溶细胞素可引心血管损伤，目前没有快速有效的手段进行检测、防护和治疗。本项目在前期分离到一种新型海葵溶细胞素GT-4及已筛选出多种海洋生物毒素适配体的基础上，拟建立一种以生物学功能为导向的适配体筛选策略并进行毒素检测及防治的研究。. 本研究首先以重组表达并纯化的GT-4为靶标，利用低浓度的GT-4能结合红细胞(RBC)膜的特性，以红细胞悬液模拟复杂的筛选体系，筛选出特异性的、高亲和力的并具有拮抗功能的GT-4适配体，将其作为识别元件，结合芯片及生物膜干涉技术技术制备免标记传感器，构建新颖、灵敏、快速的GT-4检测方法。同时从细胞水平和动物体内开展适配体拮抗GT-4毒性的功能研究。. 本项目的开展不仅能建立基于功能-SELEX的适配体筛选策略，同时建立基于生物传感器的快速检测GT-4的方法，得到的适配体还具有开发为药物的潜力，对于海葵蛰伤的防护和治疗提供先导化合物。

Cardiovascular damage can be caused by cytolysin when people were stung by the sea anemone , but there is no effective strategy for detection, prevention and treatment. Our group has isolated a novel cytolysin GT-4 and selected several of aptamers tageted marine toxins. This project is ready to establish a biological function-SELEX strategy, and develop a rapid detection method for GT-4 based aptasensor.. In this study, specific and high-affinity ssDNA aptamers binding to GT-4 will be screened in red blood cell suspension by function-SELEX strategy with low concentration of GT-4 could bind to erythrocyte membrane. As a recognition element, it will be used to combine bio-layer interferometry and label-free aptasensor to establish a novel, sensitive and rapid detection method for GT-4. While, the antagonize application of aptamers to GT-4 will be analyzed at the cellular level and in vivo.. This project will not only establish a function-SELEX strategy for GT-4, but also provide a novel, sensitive and rapid detection methods for GT-4. Aptamers have the potential to develop as a drug, which also provides a new strategy for the prevention and treatment of GT-4 poisoning.