过氧化物还原酶 1（Peroxiredoxins，Prx1）是新定义多功能特异性巯基类抗氧化蛋白，与一些疾病的发生密切相关。目前有关Prx1与烟草相关口腔白斑发生相关性研究未见报道。前期研究首次发现Prx1、烟碱型乙酰胆碱受体（nAChR）在口腔白斑中表达增高;Prx1具有抗凋亡作用；尼古丁诱导口腔癌细胞Prx1表达增高，提示Prx1可能与烟草相关口腔白斑发生密切相关。本课题拟从人口腔白斑组织、口腔癌前病变细胞株、Prx1敲除鼠胚胎成纤维细胞及Prx1基因敲除小鼠口腔癌前病变动物模型三方面，采用Q-PCR、免疫组化、ChIP、Co-IP结合质谱分析等技术，研究烟草相关口腔白斑中nAChR、Prx1作用及其相互作用，明确Prx1结合蛋白、Prx1调控凋亡相关关键下游基因及MAPK信号通路作用，从而揭示nAChR/Prx1轴在烟草相关口腔白斑细胞凋亡中作用的分子机制，为口腔白斑防治提供理论依据

Peroxiredoxin 1 (Peroxiredoxins, Prx1), as a new multi-function thio-specific antioxidant protein, plays an important role in many diseases. At present, the correlation Prx1 to tabacoo related-oral leukoplakia has not been reported. In our previous study, for the first time，the Prx1 and nAChR overexpression were detected in oral leukoplakias, Prx1 was up-regulated by nicotine in Tca8113 cells, suggesting that Prx1 may be correlated to the pathogenesis of tobacco-related oral leukoplakia. In this study, The roles of nAChR and Prx1,the interaction of Prx1 and nAChR ,Prx1 binding proteins were detected in the tissues of human oral leukoplakia, oral precancerous cell line DOK cells, Prx1 knockout mice, Prx 1-/- embryonic fibroblast cells using Q-PCR, immunohistochemistry, Co-IP, ChIP assay. The aim of the study is to investigate the roles and the molecular mechanism of nAChR/Prx1 axis in the cell apoptosis of tobacco-related oral leukoplakia so as to provide the theory basis for the prevention and treatment of oral leukoplakia.