血栓疾病发病率高，危害重。溶栓是治疗血栓性疾病的有效手段。目前溶栓药物仍存在诸多缺点。筛选、发现和研究新的溶栓药物具有重要的意义和价值。蛇毒是现代抗栓溶栓新药研究的重要资源库。本项目前期从国产蛇毒中获得一个能在体内高效溶栓并能有效抑制血小板聚集的蛋白。动物实验表明，目标蛋白在低剂量时即能有效溶栓，效果优于阳性对照尿激酶和rt-PA。其高效溶栓的作用是通过诱导内源性t-PA释放而实现。目标蛋白溶栓机制新颖，具备高效溶栓和有效抑制血小板聚集的双重作用，有可能成为有潜力的新型候选溶栓药物。本项目旨在通过开展目标蛋白体内高效溶栓的药理学及机制研究，科学评价目标蛋白的溶栓作用，阐明其新颖的溶栓机理，为目标蛋白的成药性评价提供科学依据，并有可能为溶栓新药的研究提供物质基础，同时，能促进对目标蛋白结构与功能及生物学意义的认识和理解，为相关蛇伤防治策略提供有价值的思路和参考。

Thrombosis remains a major cause of death and disability worldewide. Thrombolytic therapy is an effective approach to thrombosis. Currently, thrombolytic agents still have some shortcoming. Thus the screening and researches for new thrombolytic agents has significant meaning and value. Snake venoms are important resourses for new anti-coagulant and thrombolytic drugs. In our preliminary work, a protein was purified from snake venom which exhibited highly active thrombolysis in vivo and effective inhibition of platelet aggregation. The protein showed effective thrombolitic ability when low dose was just used. Its thrombolitic ability is better than those of urokinase and rt-PA. This highly active thrombolysis of the protein is based on inducing release of endogenous t-PA. The protein possesses novel mechanism of highly active thrombolysis, and effective inhibition of platelet aggregation. These merits make it possible to be a potential candidate for novel thrombolytic drug. The pharmacology and mechanism studies of highly active thrombolysis in vivo of the protein will be performed in this project. The thrombolisis and its novel mechanism will be elucidated and evaluated scientifically. The results will provide some helpful reference for evaluating the protein as a potential thrombolytic candidate. And it is possible to provide potential candidate to developing new thrombolytic drug. It also may give a better understanding about the structure and function of the protein and its biological meaning, and may provide some helpful clues for new strategy in the prevention and treatment of the related snake bite.