ChIP-seq是研究蛋白质与DNA互作和表观遗传学的常规技术，广泛用于全基因组转录因子结合位点和组蛋白修饰分析。目前在植物领域通用的ChIP-seq方法，起始样品用量大、效率低，而且建库成本高，限制了其在植物中的广泛应用。本项目拟建立植物通用的ChIP-seq新技术eChIP-seq。初步结果显示，新技术样品用量少、成本低、简便高效，可以为植物表观遗传学和分子生物学研究提供技术支持。本项目应用eChIP-seq方法，综合分析甘蓝型油菜两个品系的5种组蛋白修饰及RNAPII结合位点，并结合DNA甲基化，构建表观基因组图谱，分析不同组织的染色质状态动态变化，整合相应的转录组数据，解析品系间遗传变异控制表观遗传修饰和基因表达的机制，拓展甘蓝型油菜功能基因组学研究，为其他多倍体植物的表观遗传学研究提供借鉴。

ChIP-seq (chromatin immunoprecipitation followed by sequencing) is a powerful technology to study epigenetics and interactions between proteins and DNA, and has been widely used for profiling genome-wide binding sites of histone modifications and transcription factors. However, the ChIP-seq methods currently used in plants are high input, inefficient and costly, limiting its application in plants. This project intends to establish a novel plant efficient ChIP-seq (eChIP-seq) technology, which is low sample, low cost and effective, and could provide powerful technology for the research of plant epigenetics and molecular biology. Through comprehensive analysis of DNA methylation, histone modifications, binding sites of RNAPII, and transcriptome data from rapeseed (Brassica napus), we construct its epigenomic map, analyze the dynamic changes of chromatin states from distinct tissues, and uncover the mechanism of epigenetic and gene transcription regulated by genetic variations between two lines. This research not only provides a theoretical basis for rapeseed functional genomics, but also provides a reference for the epigenetic studies of other polyploid plants.