基因LOV1参与浒苔响应高温胁迫的分子机制
结题报告
批准号:
42106099
项目类别:
青年科学基金项目
资助金额:
20.0 万元
负责人:
何渊
依托单位:
学科分类:
生物海洋学与海洋生物资源
结题年份:
2023
批准年份:
2021
项目状态:
已结题
项目参与者:
何渊
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中文摘要
在诸多的环境因素中,温度是影响浒苔生命活动的最重要气候因子。在夏季,浒苔会遭遇高温等不良环境,而夏季却是浒苔爆发的时期,说明浒苔必定具有特殊的生态适应机制来应对高温环境的胁迫,但是目前缺乏对浒苔耐高温机制的系统研究。申请人前期发现高温处理对浒苔的生长和基因表达谱有重要影响,通过酵母高通量筛选技术已获得浒苔耐高温基因LOV1,推测LOV1通过增强浒苔抗氧化能力来适应高温的不利环境条件。本项目拟通过浒苔瞬时转化、拟南芥稳定转化和酵母过量表型验证技术以验证LOV1的耐高温效果,通过构建酵母双杂文库和GST融合蛋白沉降技术来阐明LOV1响应高温胁迫的互作网络,以期深入研究LOV1调控浒苔适应高温的分子机制。通过本项目的研究,完善了对浒苔耐高温过程的认识,有利于最终解析浒苔爆发的机制。
英文摘要
Temperature is the most important climatic factor affecting the life activities of Ulva prolifera among many environmental factors. In summer, U. prolifera encounters bad environments such as high temperature, while summer is the period when U. prolifera erupts, indicating that U. prolifera has a special ecological adaptation mechanism to cope with the stress of high temperature environment, but currently there is no systematic study for the high temperature resistance mechanism of U. prolifera. The applicant found that high temperature treatment has an important effect on the growth and gene expression profile of U. prolifera. Through high throughput screening of yeast technology, a high temperature resistance gene LOV1 has been obtained from U. prolifera. It is speculated that LOV1 can adapt to high temperature by enhancing its antioxidant capacity. This project intends to verify the high temperature resistance effect of LOV1 through transient transformation of U. prolifera, stable transformation in Arabidopsis and verification technology of excessive phenotype of yeast. This project intends to clarify the interaction network of LOV1 in response to high temperature stress by constructing a yeast two-hybrid library and GST pull-down technology, in order to further to study the molecular mechanism of LOV1 regulating U. prolifera to adapt to high temperature. Through the research of this project, the understanding of the high temperature resistance process of U. prolifera will been improved, which is helpful for the final analysis of the mechanism of U. prolifera eruption.
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