动粒是一个复杂的功能性蛋白质复合物，在有丝分裂过程中，为姐妹染色单体的准确分离提供必要条件。它的组装随细胞周期的进展可能会出现多个中间体，而研究这些中间体复合物的结构，对明确动粒组装机制而言不可或缺。. 本项目拟以人源CENP-A核小体/CENP-CLNHIKM复合物为研究对象，利用结构生物学、生物化学和细胞生物学方法，解析其高分辨率结构，阐明其在细胞有丝分裂过程中的功能，揭示其在动粒装配和在维持基因组稳定性过程中发挥的重要作用，为新型有丝分裂抑制剂的研发提供理论支持，也为治疗由非整倍体引起的疾病提供可能的靶点。

During mitosis, sister chromatids are precisely divided into two daughter cells to complete the transmission of genetic material, which is crucial for the healthy growth and development of organisms. The first condition for the accurate separation of sister chromatids is the correct attachment of spindle microtubules to centromeric-chromatin dependent on kinetochore. Kinetochore is a large functional protein complex, whose assembly on centromere is step by step as the development of cell cycle. In the process, many different intermediates may arise. However, the study of these intermediate complexes is essential to elucidate the mechanisms of kinetochore assembly. The specific recognition of CENP-N and CENP-C for CENP-A nucleosome and the recruitment of CENP-L/HIKM are crucial for kinetochore assembly.. In this project, we want to know the structural and functional mechanisms of CENP-A nucleosome/CENP-CLNHIKM complex in kinetochore assembly and how its regulation in cell mitosis. The result will reveal its important role in the assembly of kinetochore and the maintenance of genomic stability, and to provide a possible target for the treatment of related diseases.