高含固厌氧消化是污泥厌氧处理技术发展的方向之一。由于高含固条件下其厌氧消化液的有机物、氨氮、悬浮物以及未知抑制性组分的含量均远高于传统厌氧消化液，给基于厌氧氨氧化的高效自养脱氮过程带来新挑战。本研究聚焦高含固污泥消化液高效低耗自养脱氮的现实需求，解析高含固污泥消化液中自养脱氮抑制性组分特征，尤其是通过多种手段识别表征抑制性未知组分；分类探究以胁迫性碳源为代表的各组分在阻碍传质、产生毒害、引发竞争等方面的抑制作用机制，分析单/多因子联合作用下微生物压力响应特性，揭示抑制因素作用模型及抑制阙值；探讨预曝气和同步亚硝化反硝化两种基于胁迫性碳源去除的调控策略，探究缺氧/好氧环境下各抑制性组分的代谢规律，基于反应器运行及分子生物学手段揭示不同策略的调控效果及功能微生物群落特征。最终为突破我国高含固污泥厌氧消化液高效自养脱氮瓶颈问题提供科学依据和技术基础。

High solid sludge anaerobic digestion (AD) achieved more and more attentions. However, the digester supernatant from high solid AD generally demonstrated higher COD, higher ammonia nitrogen, higher suspended solids and higher unknown inhibitory components than that of traditional AD, which brings about new challenges to the high-efficiency autotrophic denitrification process based on anaerobic ammonia oxidation. In this study, it is planned to systematically analyze the concentration and characteristics of inhibitory components such as organic matter, ammonia nitrogen and suspended matter in high solid digestion supernatant and especially to identify the unknown inhibitory components by various means. Also, their effects on mass transfer, toxicity and heterotrophic bacteria competition should be explored with investigation of microorganisms response under single / multiple factors. The inhibition model and threshold should be revealed. It was proposed that two strategies of pre-aeration and simultaneous nitrification & denitrification to enhance nitrogen removal. The metabolic pathway of inhibition component in the anoxic / aerobic environment was explored and the characteristics of functional microbial community was uncovered based on reactor operation and molecular biological analysis. In general, this study was expected to provides scientific basis and technical support for breaking through the bottleneck of high-efficiency autotrophic denitrification of high solid sludge digestion supernatant in China.