捕食线虫真菌是自然界中控制线虫种群数量的重要因子，也是动植物线虫病害生物防治的重要研究材料。捕食器官是真菌捕杀线虫的重要武器，也是捕食线虫真菌从腐生转变为寄生生活的重要标志。前期研究发现，部分氨基酸能诱导捕食线虫真菌产生捕食器官，然而相关的分子机制未见报道。本项目以捕食线虫真菌寡孢节丛孢作为研究对象，筛选能有效诱导寡孢节丛孢产生捕食器官的氨基酸；通过转录组技术和Real time PCR技术比较在氨基酸诱导和非诱导条件下寡孢节丛孢的基因表达差异，确定参与氨基酸诱导真菌产生捕食器官的候选基因；通过基因敲除和互补实验进一步鉴定候选基因的功能，确定参与氨基酸调控寡孢节丛孢形成捕食器官的关键基因和相关生物学途径。本项目研究结果不仅为揭示捕食线虫真菌形成捕食器官的分子机制奠定基础，同时也为提高捕食线虫真菌捕杀线虫的能力和开发高效线虫生防制剂奠定基础。

Nematode-trapping fungi play important roles in maintaining nematode population density in natural environments, and they are important materials for biological control of animal and plant disease caused by nematodes. Trapping devices (traps) are the important tools for fungi to capture nematodes, and the formation of traps by nematode-trapping fungi is an important indicator of their switch from the saprophytic to the predacious lifestyles. Previous studies found that several amino acids can induce the nematode-trapping fungi to produce traps, while little is known about the molecular basis of amino acids regulate the trap formation in the nematode-trapping fungi. In this study, the fungus Arthrobotrys oligospora, a typical strain of nematode-trapping fungi, was used to screen amino acids which could induce the trap formation effectively, and identify the candidate genes involved in the regualtion of trap formation in A. oligospora under induction conditions by amino acids using transcriptomic and Real time PCR analyses, and reveal the biological roles of candidate genes in A. oligospora using gene knockout and complementary experiments, and identify the genes and related pathways involved in the regulation of trap formation during induction process by amino acids. Our results will provide a basis to elucidate the molecular mechanism of trap formation, improve the nematicidal activity of nematode-trapping fungi and develop more targeted anti-nematode agents.