携表型遗传标记水稻单倍体诱导系的创制与应用

加倍单倍体技术能快速培育遗传纯合的材料，在作物育种中具有广泛的应用前景。创制具有表型遗传标记的单倍体诱导系将有助于单倍体的快速筛选，大大提高单倍体的育种效率。本项目通过对水稻种质资源的筛选鉴定，获得密绒毛叶、紫色茎秆和斑点叶等具有显性表型标记的遗传材料。在此基础上，利用基因组编辑技术突变OsMTL基因，筛选获得无转基因成分、携带表型遗传标记的单倍体诱导系。并且通过杂交、自交方法建立多抽穗期的携表型遗传标记水稻单倍体诱导系，从而加速加倍单倍体技术在水稻育种中的应用。

Doubled haploid (DH) technology offers the fastest route to produce homozygous inbred lines, which is full of great application values in crop breeding programs. Development of phenotypic markers-based haploid inducers will speed up the identification of haploids from diploids, thereby improve the efficiency of haploid breeding. Here, we obtained genetically dominant materials with dense velvet leaf (DVL), red stem (RS) and spotted leaf (SL), respectively, through screening of rice germplasm resources. We plan to knockout OsMTL gene in these materials to generate haploid inducers with phenotypic markers by genome editing technology and screen lines without T-DNA elements, and investigate their haploid induction rate as well. Then we will develop phenotypic markers-based haploid inducers with multiple heading date by crossing and selfing for further applying them in rice breeding.