细胞质雄性不育(CMS)是作物杂种优势利用的基础，本课题组率先发现并选育了红麻CMS系，红麻杂种优势利用已取得突破性进展，但关于红麻CMS发生的机制研究尚鲜见报道。本研究以红麻细胞质雄性不育系和保持系为材料，分别分离其花药mRNA，利用最新的RNA-seq技术对其花药进行转录组比较研究。分别对差异表达的基因进行GO功能注释和富集分析，并进行KEGG代谢通路分析，研究其调控和代谢网络，通过比较差异表达的基因，发掘与CMS相关基因。运用 RT-PCR及Northern blot的方法进一步验证差异表达基因，重点克隆与CMS相关的关键基因。采用农杆菌介导进行转基因研究，超表达或者沉默相关基因，通过后代育性分析评价基因功能。结合本课题组目前的基因组和差异蛋白质组研究结果，可望揭示红麻CMS发生的分子机制，从而促进红麻杂种优势的利用进程，同时也为其它植物CMS机理的揭示提供基础性资料。

The cytoplasmic male sterile (CMS) lines played a crucial role in utilization the heterosis of crops. To date, a breakthrough has been made in kenaf CMS lines breeding and heterosis utilization, but little is known about the mechanism on the molecular basis of CMS. In the present study, the anthers of CMS and its maintainer lines were used for transcriptomic analysis by newly reported RNA-seq strategy. The differential expressed genes will be analyzed by GO (gene ontology) annotation and KEGG（Kyoto Encyclopedia of Genes and Genomes）pathway analysis. Then construct the regulatory and metabolic networks, and discovery the CMS related genes. Some of the CMS related genes will be cloned after RT-PCR and Northern blot verification. The key CMS involved genes will be silenced or over-expressed in kenaf to evaluate their function by Agrobacterium mediated transformation. The result, combined our present research, should reveal the mechanism of kenaf CMS and promote the kenaf heterosis utilization. Furthermore, it may give some clues in elucidating the CMS of other crops.