成骨障碍是导致激素性股骨头坏死（SONFH）预后不良的关键原因，活血通络法（HXTL）是中医促进SONFH骨修复的可靠方法。FUT8介导的核心岩藻糖基化（CF）修饰是重要的转录后修饰，前期研究首次发现SONFH中CF修饰显著降低与骨修复障碍相关。我们推测CF修饰降低可通过抑制BMSCs成骨分化阻碍SONFH骨修复，而FUT8也可能是HXTL法的作用靶点。为验证该假说本研究首先通过临床研究，使用iTRAQ-TOF质谱及RNA-Seq分析SONFH中CF修饰靶点；并通过体内外实验，使用Crispr/cas9，慢病毒转染，免疫荧光素酶报告，免疫沉淀，Lectin blot等方法分析CF修饰对BMSCs介导SONFH骨修复的影响以及活血通络中药的干预机制，为中医药治疗SONFH寻找新的靶点与理论依据。

Impaired osteoblast differentiation is a critical factor for the poor prognosis of SONFH, the traditional medical method of HXTL is reliable to treat this disease. FUT8 mediated core fucosylation is an important posttranscriptional modification, our previous study firstly found that CF decreased significantly and impaired bone repair in SONFH. We speculated that decreased CF would suppress osteoblast differentiation, meanwhile FUT8 may be a potential target of HXTL method. To test this hypothesis, iTRAQ-TOF mass spectrometry and RNA-seq will be used to analyze the targets of CF in clinic research. And crispr-cas9, lentivirus overexpressing, lectin blot, immunoprecipitation and luciferase reporter will be used to explore the effect of FUT8 mediated CF on osteoblast differentiation and the mechanism of HXTL in vitro and vivo. Our work will contribute to find a new mechanism of Chinese traditional medicine improving impaired bone remodeling in SONFH.