实现对脱氧核酶结构和性能的精确调控是一个研究热点和难点。电化学方法是一个简便的、重要的研究工具和调控手段，有利于实现对核酸分子的准确结构调控。本课题拟采用电化学方法调控核酸分子的结构和金属离子的价态及空间分布等，揭示电化学激发条件下金属离子与核酸分子之间的相互作用规律。采用静电场调控方法研究脱氧核酶分子在固/液界面处的构象变化和催化性质改变；采用电化学氧化还原法调控金属离子的价态，进而影响溶液相脱氧核酶分子的结构和性质；发展电化学选择性激活多脱氧核酶体系。揭示不同电化学条件下脱氧核酶与底物的作用机理，为脱氧核酶结构及性能的电化学调节方法提供理论依据，尝试将电化学激发脱氧核酶用于制备一维核酸纳米结构、调控核酸纳米机器等。作为一种普适性电化学调节方法，相信随着生物技术的发展，将会在电化学治疗技术等更多领域内得到更广泛应用。

Precise regulation of DNAzyme by changing its structure and properties attracts more and more attention in the field of DNA nanotechnology. Electrochemistry is a simple and important research tool to fulfill this job. It has been used as a powerful regulation means to control the structure of nucleic acid. Here we would like to apply electrochemical methods to control the structures of nucleic acids, to regulate the valence states of metal ions, to manipulate the spatial distributions of nucleic acids and metal ions, and to study their interactions. First, electrostatic field was used as a regulation tool to control the conformational and catalytic properties of DNAzyme molecules immobilized on the electrode surface. Then, electrochemical redox regulation of the valence state of metal ions can be used to study the structure and functions of the DNAzyme in aqueous solution. This method can be extended to selectively regulate the multiple DNAzyme systems. Based on the aforementioned results, we would like to reveal the interaction mechanisms of DNAzymes and their substrates by different electrochemical stimuli. These results can provide a theoretical basis for electrochemical adjusting the activities of DNAzymes. Finally, electrochemical stimulating method can be used for the preparation of one-dimensional biomolecular nanostructures, to regulate nucleic acid nanomachines and so on. As a universal triggering stimuli, we believe that with the development of biotechnology, electrochemical method will play an important role in the field of clinical electrochemical treatment technology.