原发性肝癌（Hepatocellular Carcinoma，HCC）是最常见的恶性肿瘤之一，早期诊断技术的滞后是其防治的重点和难点。课题组前期研究发现，目前基于传统检测方法的miRNAs和lncRNA分子诊断方法，依然存在灵敏度低和特异性差等缺点。现有研究证实，circRNA在肿瘤组织中比miRNA和lncRNA更加特异和稳定。为此，本课题拟采用circRNA芯片筛选鉴定HCC的特异性circRNAs，并构建新型肝癌电化学SHERLOCK检测系统。与传统检测方法相比，该系统基于sgRNA 引导的Cas13a核酸酶可以精准侧切割circRNAs分子中的靶向位点；基于Cas13a/Csm6重组聚合酶信号放大方法，可以检测到阿摩尔级（10-18 mol/L）浓度的核酸分子。研究结果将筛选出若干个HCC差异性circRNAs，并为HCC的超灵敏、高特异性早诊早治提供新的策略。

Hepatocellular Carcinoma (HCC) is one of the most common malignant tumors, and the development of early diagnosis technology is the key and difficult for its prevention and treatment. In our previous research, we found that traditional detection methods of the miRNAs and lncRNA still have shortcomings such as low sensitivity and poor specificity. Currently studies have confirmed that the circRNA is more specific and stable in tumor tissues than miRNA and lncRNA. Therefore, we would use the circRNA chip to screen and identify the specific circRNAs of HCC. In additional, we would construct a new SHERLOCK detection system. Compared to the traditional detection method, the system of Cas13a nuclease based on sgRNA guide could the precise collateral effect to target of circRNAs, and the detection signal amplification to 10-18 mol/L based on the recombinase polymerase amplification（RPA）of Cas13a/Csm6. In this project, the differential circRNAs of the HCC will be screened. In aditional, the ultrasensitive, highly specific, and fast detection method of electrochemistry SHERLOCK system data will be construct, which is crucial for the fast diagnosis, prevention and control of HCC.