单纯疱疹病毒（HSV）是重要的人类致病病毒，针对目前临床治疗药物单一及不断出现的耐药现象，申请者以中药余甘子活性成分PGG为研究对象，探索其抗病毒的分子机制。在我们的前期工作中，首次发现PGG抑制核质边缘化和核骨架减少从而抑制病毒粒子出核；蛋白质组学研究和Western-blot检测发现PGG下调细胞骨架蛋白Cofilin1。因此，我们推测PGG的抗病毒作用与其对细胞骨架微丝系统的调控作用存在一定相关性，并通过体内、外实验继续探讨PGG对HSV-1病毒进/出细胞核的影响以及对细胞骨架蛋白尤其是Cofilin1的影响，为同类中药的抗病毒机制研究提供参考。

Infection by herpes simplex virus (HSV) is a global public health problem. Although acyclovir (ACV) and related nucleoside analogs are valuable drugs for the treatment of infection and the suppression of dissemination, HSV remains highly prevalent worldwide. The emergence of resistance to ACV has also created an obstacle for the treatment of HSV-infected immunocompromised patients and highlights the need for new, safe and effective antiviral agents. Natural products, particularly the traditional Chinese medicines, which provided various novel drug leads, are known to be an important source of anti-HSV agents. Here we will investigate the underlying mechanism of anti-herpes simplex virus type 1 activity of pentagalloylglucose (PGG) from Phyllanthus emblica Linn. Our previous works found that PGG inhibited HSV-1-induced destruction of cytoskeleton integrity which is related to the reduction of F-actin and of G-actin, and blocked HSV-1 capsids egress from nucleus to cytoplasm. Besides, PGG can downregulate Cofilin 1 and inhibit HSV-1-induced rearrangements of actin cytoskeleton which is important for infectivity. Thus, to analyse the relationships between host cell cytoskeleton, especially the protein Cofilin 1, and anti-HSV activity of PGG, a better mechanistic investigation paradigm may arise.