寨卡病毒（ZIKV）所诱导的CD8+T细胞反应具保护作用但也可能参与疾病的发生。通过生物信息学分析和预实验，我们发现ZIKV感染所诱导的CD8+T细胞可交叉识别宿主抗原肽。我们拟首先检测在ZIKV感染小鼠时，CD8+T细胞浸润小鼠重要器官（大脑、脊髓、睾丸）和外周神经情况；然后通过生物信息学分析选择合成ZIKV特异性CD8+T细胞表位在人/小鼠中的具有相似氨基酸序列的宿主抗原肽；评估ZIKV/宿主交叉反应性CD8+T细胞体外交叉识别宿主抗原肽、杀伤宿主细胞效应；探讨ZIKV/宿主抗原肽交叉反应性CD8+T细胞突破血脑屏障的能力及进入屏障组织内杀伤宿主细胞的效应；最后研究预存在的ZIKV/宿主抗原肽交叉反应性CD8+T细胞在ZIKV感染时对组织中ZIKV病毒滴度、组织器官病变的作用。研究成果将为安全有效的ZIKV精准疫苗（剔除或突变与宿主抗原肽具有交叉反应的表位序列）研发提供新的策略。

Zika virus (ZIKV)-specific CD8+T cells have protective function and may be associated with the pathogenesis of zika diseases. Through bioinformatics analysis and performing pre-experiments, we found that ZIKV-induced CD8+T cells can cross recognize the host antigenic peptides. In the present project, we will detect CD8+T cell infiltration of important organs (brain, spinal cord and testis) and peripheral nerve of ZIKV-infected mice; then choose and synthesize human/mouse host antigenic peptides with similar amino acid sequence with ZIKV-derived CD8+T cell epitopes by bioinformatics analysis; evaluate the ability of ZIKV/host cross-reactive CD8+T cells to recognize host antigenic peptides and kill host cells; explore the ability of ZIKV/host cross-reactive CD8+T cells to break through the blood-brain barrier, blood-testis barrier and invade the barrier tissues to kill host cells; and finally investigate the effect of pre-existing ZIKV/host cross-reactive CD8+T cells on ZIKV viral load in tissues and pathological changes of tissues and organs. This research will provide a new strategy for the development of the safe and effective ZIKV precise vaccines, which delete or mutate the sequence of ZIKV/host cross-reactive epitopes.