肝癌的早期诊断是目前治疗的关键，寻找一种具有高特异性、敏感性及操作简便的检测方法是实现肝癌早期诊断的关键，本课题组利用前期构建的纳米生物传感器成功检测了溶液中极低水平的IL-6，并通过优化纳米生物传感器与蛋白质的连接，增加钝化层厚度，改进微流道结构，成功的联合检测到溶液中pg/mL?1水平的AFP、AFP-L和IL-6较稳定的电信号。若进一步分别检测PHC患者、正常人群、乙肝肝硬化患者、慢性肝病患者与PHC相关的肿瘤标志物各自表达的特点，并筛选出在pg/mL?1水平与PHC相关的肿瘤标志物，再次联合检测具有不同转移能力的PHC细胞株，将整个观测频谱范围内不同频率峰值电信号采用傅里叶转换，贝叶斯概率统计，分离盲源，排除干扰，使不同转移能力的肝癌细胞株表现出不同的传导信号。这些独特信号，可构建具有高敏感性和强特异性的PHC肿瘤标志物的临床诊断图谱，有望提供通过一滴血的检测实现对PHC早期诊断。

Early diagnosis is the key to treat the liver cancer. Looking for a high specificity, sensitivity and simple detection method is one of the most important points to the early diagnosis of liver cancer. A nanosensor made of magnetic nano particles, which can be considered as my group's pre-achievement, has been discovered by my group to be available to detect ultra-low level IL-6. At the same time, we used the DCS method to connect the antigen to the SiNW, improve the connection of microfluidic system, and increase the thickness of the passivation layer of the nanosensor. We successful detected AFP, AFP-L3 and IL-6 with the new nanomedical system in BSA solution simultaneously. So, the sensor will be continuously be used to detect the sign character of PHC tumor marker in PHC patients, normal people, hepatitis B cirrhosis and chronic liver disease patients. Screening the markers in pg/mL ? 1 levels associated with PHC.Some studies will be made on the relationships between sensor's resistance and concentration of tumor markers. The observed spectral range of different peak frequency signal by using Fourier Transform, Bayes probability and statistics. By separated blind source, eliminated interference, the hepatocellular carcinoma cell lines with different metastatic ability will show different signal conduction. These unique will provide a system to early decet the PHC which is high sensibility and strong specific, also maybe detcet PHC tough only a drop of blood.