活性氧在银屑病患者血小板高活性状态中的作用及白藜芦醇干预研究

银屑病患者血小板处于高活性状态，易于形成血栓。活性氧（ROS）和炎症在银屑病的发生发展过程中起重要作用，ROS是否参与银屑病患者血小板高活性状态目前未明。申请者最新研究发现，银屑病患者血小板发生活化和凋亡，血小板活化和凋亡可能与血小板高活性状态密切相关；同时发现，银屑病患者血小板胞内ROS和线粒体ROS浓度升高、聚集功能增强，ROS拮抗剂降低血小板聚集功能，提示ROS可能在银屑病患者血小板高活性状态中起重要作用。白藜芦醇具有抗氧化、抗炎症和抑制血小板聚集等多种生物学功效，我们推测白藜芦醇可能改善血小板高活性状态。围绕这些发现，本课题拟进行以下研究：1、全面证实ROS在银屑病患者血小板高活性状态中的作用；2、探讨ROS引起血小板高活性状态的分子机制；3、探讨白藜芦醇改善血小板高活性状态的分子机制。预期结果有助于进一步认识血小板高活性状态的分子机制；同时为白藜芦醇应用于银屑病治疗提供新的视角。

Hyperactive platelets of patients with psoriasis could easily form thrombus. Reactive oxygen species (ROS) and inflammation play important role in the progression of psoriasis, however, whether ROS is involved in forming hyperactive platelets in psoriatic patients is unknown at present. Our preliminary experiment showed that activation and apoptosis of platelets in psoriatic patients may be closely related to hyperactivation of platelet. Increased concentrations of intracellular and mitochondrial ROS and enhanced aggregation in platelets were happened, which suggested that ROS play a key role in the formation of hyperactivation of platelets in psoriatic patients. It was speculated that resveratrol could change the status of hyperactivation of platelets, as it has various biological functions, such as anti-oxidant and anti-inflammatory and inhibiting platelet aggregation. According to the above findings, we plan to in-depth study, including (1) confirming this hypothesis that ROS participates in platelet hyperactivation in psoriatic patients; (2) exploring the mechanism of platelet hyperactivation caused by ROS; (3) studying the molecular mechanism of improving the situation of hyperactivation of platelets by resveratrol. The expected results would help to further understand the molecular mechanisms of platelet hyperactivation and to provide a new perspective about the treatment of psoriasis used resveratrol.