蛋白质相互作用是细胞中功能调控的基础，研究与疾病相关蛋白质之间的相互作用对了解疾病的发生发展具有重要意义。α-syn为天然无序蛋白，在不同环境下存在结构差异。研究表明，α-syn可与synaptobrevin-2（syb2）发生相互作用参与神经递质释放调节，该过程与帕金森病发生密切相关，然而α-syn与syb2相互作用的分子机制目前尚不清楚。本项目将采用核磁共振波谱技术分别在水环境、膜环境以及细胞内拥挤环境中研究α-syn与syb2的相互作用。具体包括：1.分析相互作用是否依赖于环境诱导的蛋白结构；2.比较不同环境中相互作用的热力学性质；3.探究相互作用界面。通过本项目的研究探寻环境效应对天然无序蛋白相互作用的影响，研究结果将对揭示α-syn与syb2在细胞中相互作用的分子机制、了解α-syn调节神经递质释放机理以及研究帕金森病致病过程都有重要意义。

Protein-protein interactions are the basis of functional regulations in cells. It's significant to study the interactions of the proteins for the knowledge of the occurrence and development of relevant disease. α-syn is an intrinsically disordered protein, which has different conformations in different environments. Recent observations indicated that α-syn participate in the regulation of neurotransmitter release through directly binding to synaptobrevin-2 （syb2）, and this process is closely related to Parkinson's disease. However, the molecular mechanism of the interaction between α-syn and syb2 is unknown till now. This project is going to investigate the interaction between α-syn and syb2 in different environments, including in aqueous buffer, in membranes and in cells using the nuclear magnetic resonance (NMR) spectroscopy, and will reveal the relationship of the protein interactions and protein conformations in different environments. Furthermore, the thermodynamic property and the binding surface involved in the interaction between these two proteins will also be studied. Through this project, we will explore the role of the environment in the interactions between intrinsically disordered proteins. This research will reveal the molecular mechanism of the interaction between α-syn and syb2, and make knowledge of the role of α-syn in the regulation of neurotransmitter release, which are of important significance for the study of the Parkinson's disease.