内病外治穴位贴敷药物以远小于口服剂量作用于穴位，在全血中很难找到药动学规律。据此，课题组认为中药有效成分是通过穴位吸收，形成局部药物贮库效应，达到局部较高的药物浓度，通过药物贮库应答产生经穴放大效应，从而发挥相对较强药理作用。拟选取"中药青藤碱外敷散"作为模型药物穴位给药，采用微透析技术对类风湿性关节炎模型动物进行穴位、关节腔和与血液定时地同步取样，测定有效成分青藤碱和关节腔内精氨酸、瓜氨酸、IL-1、IL-4等含量变化，建立PK/PD模型；并与口服该药PK/PD关系比对，寻找出穴位给药奏效的关键影响因素及作用机制；并采用荧光内窥式激光共聚焦技术，观察记录荧光标记后的药物在穴位组织的吸收、分布和消除过程，旨在通过经皮渗透特性、穴位效应及PK/PD相关性研究，阐释中药外敷散穴位给药内病外治作用机理，揭示穴位吸收与整体效应关系，为中药复方穴位贴服药物内病外治机理研究奠定基础。

The Acupoint Plaste doses is far less than the oral doses,and it is hard to find pharmacokinetic rule in whole blood.Therefore, we propose that Chinese medicine effective component is absorbed by acupoint, forming local body drug library storage effect, and achieve high local concentration of the drug. The drug storage response has meridians amplification effect, so as to improves pharmacological action.Sinomenine power was used as a model drug,microdialysis technology was used to gather up sample in Acupuncture points, articular cavity and blood.Enzyme immunoassay was used to determined the content of IL - 1, IL - 4 in articular cavity.PK/PD model was established, between it and oral were contrasted. the key influencing factors were found out. Using fluorescent endoscopic confocal laser technology, the absorption, distribution and elimination of process were studied.We aim to not only investigate the penetration characteristics,effect in points and PK/PD, but also to explain the Acupoint Plaste doses function mechanism in order to reveal the relationship between the part and the whole in absorption.We will study the compound Chinese medicine in the future.