内源性多肽是蛋白质在体内水解酶作用下产生的多肽碎片，它蕴含着丰富的生物学信息，能够反映生物机体内与蛋白质合成和降解相关的酶活性，为我们对生命过程的认识和疾病标记物的发现提供不可或缺的资源。申请人在前期的工作中致力发展基于硅基和碳基介孔材料的内源性多肽的高效富集方法，构建了内源性多肽分析的液相色谱-质谱平台；同时还发展了原位标记、N-端选择性标记等基于二甲基化的同位素标记蛋白质组定量方法。针对目前内源性多肽规模化定性与定量方法的缺乏，本项目拟进一步发展血清中内源性多肽的高效富集技术，结合稳定同位素标记定量技术，构建集成化的内源性多肽组学定量分析系统，提高内源性肽定性定量分析的准确性、灵敏度和通量。本项目的开展实施将有效地推进我国在多肽组学定性定量分析方面的分析技术。

Endogenous peptides, as the fragments of proteins digested by multi endogenous proteases, potentially reflect an archive of important histological information whose determination could serve to improve early disease diagnosis. And the endogenous peptides in serum are the important pool of potential biomarkers for the elucidation of biological and pathological variations in the serum. The analysis of peptidome may be the bridge between the proteomics and metabolomics. In our previous works, a highly efficient extraction based on ordered mesoporous carbon materials was integrated with high through-put system for the analysis of low abundance endogenous peptides. Additionally, we also developed methods based on highly selective in-situ solid phase labeling and site-specific labeling for the quantification of peptides and proteins. As there is lack of strategy for the comprehensive identification and quantification analysis of endogenous peptides, we propose to establish methods for the highly efficient extraction of endogenous peptides, as well as fabricate integration platform for the quantification analysis of the endogenous peptides based on the stable isotope and label-free strategy. The outcome of this project will greatly improve the accuracy, sensitivity and through-put of peptidome quantification analysis.