糖皮质激素(Glucocorticoids, GCs)是临床上用于系统性红斑狼疮(Systemic lupus erythematosus, SLE)等风湿免疫病的重要药物，大剂量或长期应用会导致严重副作用，中西医结合治疗SLE可以预防和减轻激素副作用，改善患者预后。GCs引起的骨质疏松、心血管病、胰岛素抵抗等副作用与脂代谢紊乱具有一定关联，研究GCs对脂代谢的影响是阐释激素副作用发生机制的重要环节。本研究拟以脂代谢为核心，MRL/lpr小鼠为研究对象，利用基于Shotgun技术的血清脂质研究平台，对GCs治疗后小鼠血清脂质组成的时序性变化进行全面监测，获取激素相关的特征脂质谱，筛选与副作用发生发展密切相关的脂质分子，为副作用的预防和发现开拓新思路；同时考察狼疮定早期干预对激素所致脂代谢紊乱的调节作用，从脂代谢调控角度探讨中药减轻激素副作用的可能机制，为SLE的中西医结合治疗提供科学依据。

Glucocorticoids (GCs) are a powerful tool in the treatment of autoimmune diseases such as systemic lupus erythematosus (SLE).Unfortunately, high dose or long-term use of GCs often lead to numerous adverse effects, including insulin resistance, osteoporosis, cardiovascular diseases, etc. The combinination of GCs and traditional Chinese medicines seems to be an effective way to manage the side effects and improve the prognosis. Side effects of GCs is closely associated with the disorders of lipid metabolism. In the study, a MS-based shotgun lipidomics method is employed to characterize the serum lipid profile of MRL/lpr mouse treated with prednisone. And time-course variation of lipids is monitored to find out the special lipids associated with adverse effects. And the regulatory role of a Chinese compound formula-Langchuangding on lipid metabolism is investigated to elucidate the mechanism in reducing toxicity of GCs.