现代社会睡眠问题越来越突出，流行病学研究发现睡眠障碍增加了系统性红斑狼疮（SLE）等自身免疫疾病的风险。类固醇激素在SLE发生发展中有重要作用，其分泌节律受生物钟系统调控，因而我们推测睡眠障碍引起的激素节律异常可能会促进SLE发生。体内激素大部分浓度极低，且存在复杂的代谢网络，申请人特别开发了类固醇激素的全景分析方法，可以对40多种激素及其代谢物水平进行高灵敏检测，通过对睡眠剥夺（SD）模型类固醇激素及合成关键酶的分析，系统地阐明SD对激素分泌节律的影响；进一步考察SD对狼疮病情的影响，明确激素节律异常与SLE发生发展中的相关性；并探讨中药狼疮定是否通过调节钟基因的表达来改善激素分泌从而治疗SLE。本研究将进一步揭示生物钟紊乱与自身免疫疾病间的关系，同时为中医药治疗SLE提供理论依据。

Sleep problems have become a prominent issue in the world. Epidemiological studies have found that sleep disorders may increase the risk of autoimmune diseases such as systemic lupus erythematosus (SLE). Steroid hormone plays an important role in the development of SLE. The secretion rhythm of hormone is regulated by the biological clock system. So, we speculate that abnormal hormone rhythm caused by sleep disorder may promote the occurrence of SLE. Most of the hormones are extremely in low concentration and there is a complex metabolic network among steroids. We have developed a holistic method for the analysis of steroid hormones, which enables highly sensitive detection of more than 40 hormones and their metabolites. The influence of sleep disorder on steroid rhythm and biosynthesis related enzymes are measured based on the sleep deprivation (SD) model; then, the effect of SD on lupus disease activity is evaluated by the inflammatary cytokines. The relationship between hormone rhythm abnormalities and the occurrence and development of SLE is elucidated using correlation analysis. Futher, it is explored whether Langchuangding can improve the hormone secretion through regulating the expression of clock genes. The study will reveal the relationship between the disturbance of the biological clock and autoimmune diseases, and provide a theoretical basis for the treatment of SLE by traditional Chinese medicine.