针对高温甲烷发酵过程丙酸易积累、难降解，影响系统稳定运行，制约高效能源回收的问题，本项目拟从丙酸积累现象考察及其驱动因子识别为切入点，研究丙酸过量积累机制及调控技术。结合批次实验和连续中试实验，开展化学、微生物学、分子生物学分析和理论解析，深入研究水解酸化阶段的生物生态条件，判明丙酸产生的动力学机制；分析丙酸的乙酸化环境条件及种间电子传递作用，揭示其转化路径及生化原理；研究丙酸积累控制和降解促进途径，确立高温厌氧发酵过程调控的多元耦合技术策略。研究成果将为高温发酵系统优化设计和高效稳定运行提供理论和技术基础。

Due to the accumulation of propionic acid in thermophilic methane fermentation, stable performance and efficient energy recovery is hard to be achieved. In this project, the mechanism of propionic acid accumulation in thermophilic methane fermentation and technologies for process control will be systematically studied with the entry point of the phenomenon and driving factors analysis. Batch test combined with continuous pilot experiment will be employed for the mechanism studies of propionic acid accumulation associated with chemical, microbiological and molecular biological analysis. The dynamic mechanism of propionic acid production in hydrolysis and acidogenesis will be confirmed by bio-ecological environment condition analysis. The role of environmental factors and interspecies electron transfer will be investigated to identify the pathway and biochemical principle of propionic acid conversion. The multiple technologies for process control of thermophilic methane fermentation will be established based on the study of accumulation control and degradation enhancement of propionic acid. It will provide theoretical and technical foundation for optimizing the design of thermophilic digester and achieving stable performance with high efficient energy recovery.