早期断奶技术是现代养猪生产关键技术之一，但其会诱导仔猪肠道屏障的破坏，造成仔猪肠道免疫功能受损。因此，通过营养调控仔猪肠道免疫功能是动物营养学研究的热点之一。研究表明，精氨酸可有效缓解断奶应激造成的仔猪肠道屏障功能紊乱，但其固有免疫机制尚不清楚。巨噬细胞是固有免疫的重要组成部分，作为机体抗感染的第一道防线，存在极化现象（M1和M2亚型）。因此，本项目拟以仔猪肠道巨噬细胞为研究对象，运用Q-PCR和WB等技术研究精氨酸对M1和M2巨噬细胞极化的影响，运用FACS技术和体外肠上皮细胞损伤模型，探讨精氨酸调控对M1巨噬细胞吞噬活性和M2巨噬细胞肠道修复功能的影响，最后运用Co-IP和CRISPR/Cas9等技术探索其调控的信号转导机制。通过本项目实施将初步阐明精氨酸调控断奶仔猪肠道健康的固有免疫机制，有助于全面理解氨基酸营养调控动物肠道健康理论，亦为早期断奶仔猪的营养调控提供理论依据和新思路。

Early weaning technology is one of the key technologies in the modern swine industry, which induce the intestinal barrier injury and affect the intestinal immune function. Thus, regulation of piglet intestinal immune function by nutrition is a hot issue in animal nutrition research. It has been reported that arginine regulates the dysfunction of intestine in weaned piglet, while its mechanism has not been identified. Macrophages are the most important component of the innate immunity and have M1 and M2 polarization. This project intends to study the effect of arginine on macrophage polarization by Q-PCR and Western Blot technology. FACS and IPEC-J2 wound-healing model will be used to study the phagocytic activity and wound-healing function of polarized macrophage. Co-IP and CRISPR/Cas9 technology will be used to further explore the signal transduction pathway. Together, these data may clarify the innate immunity mechanism of arginine regulation function on the intestinal health and provide valuable evidence for amino acid nutritional regulation in weaned piglet production.