从植物中寻找二肽基肽酶四（DPP-IV）抑制剂是糖尿病新药研究的一个新方向。薄层色谱-生物自显影技术在天然抗氧化剂、胆碱酯酶抑制剂等方面的应用提示该技术在对成分复杂的植物药活性筛选方面具有传统分光光度法所无法比拟的优势。因此本课题拟以发现DPP-IV抑制剂为切入点，建立新的薄层色谱-生物自显影活性筛选方法，以期快速寻找和发现天然DPP-IV抑制剂，并将其与质谱技术联用，使该法集快速、简便、高通量及活性产物结构鉴定为一体。该方法的建立与应用，一方面可快速发现天然DPP-IV抑制剂，为新型降糖药物研究提供先导化合物。另一方面，也为其他天然活性成分的快速筛选和发现提供思路和方法。

It is a new direction for antidiabetic drugs research to screening natural DPP-IV inhibitors from plants. Application and advantages of TLC-bioautographic method in natural antioxidants and cholinesterase inhibitors showed that the method is better than traditional spectrophotometry, especially in plants with complicated chemical compounds. Therefore, this project intends to establish a new DPP-IV TLC-bioautographic method, in order to quickly screening and discover natural DPP-IV inhibitors, and coupled with TLC-MS technique to establish a method integrated fast, simple, high-throughput with structure identification of active compounds together. The above research will be benefit for rapid discovery of natural DPP-IV inhibitors, providing lead compounds for new antidiabetic drug research. Meanwhile, it will provide a new idea and new model for the rapid screening and discovery of other natural active ingredients.