AMPA受体离子通道型谷氨酸受体亚型之一，主要介导脑内的快速兴奋性神经传递，是神经元兴奋性突触后电流（EPSC）的物质基础。Stargazin作为第一个被鉴定的AMPA受体辅助调节蛋白(TARPs)，是近年来AMPA受体药物研发的新靶点。它能够在突触后膜上与AMPA受体相互作用，具有调节AMPA受体的生理学和药理学功能的特性。基于申请者以往的对AMPA受体和stargazin的研究基础，本课题拟针对stargazin的EX1和EX2片段进行分子生物学、结构生物学和电生理学研究，解析stargazin的功能片段，精确阐明stargazin调节AMPA受体的门控模式，优化stargazin和AMPA受体的研究方法，为研发AMPA受体调节药物提供理论及实验依据。

AMPA receptor is one of glutamate glutamate-gated ion channels that mediate fast excitatory synaptic transmission in the mammalian CNS. Stargazin as the first transmembrane AMPA receptor regulatory proteins (TARPs) found in CNS, which could regulate the physiological and pharmacological function of AMPA receptor. The discovery of stargazin points a new pathway to design AMPA receptor modulator. Here we will focus on the EX1 and EX2 fragment of stargazin to identified the functional fragment of stargazin by molecular biology, structural biology and electrophysiology. We hope our study could clarify the precised modulation of stargazin on the gating mode of AMPA receptor, optimizate strategy for study the interaction of stargazin and AMPA receptor, and provide the theoretical and experimental basis for the development of AMPA receptor modulating drugs.