近年来的研究表明趋化性细胞因子及其受体在肿瘤的转移过程中发挥着重要作用。我们的前期研究发现，CCR9在急性T淋巴细胞白血病(T-ALL)患者的白血病细胞上异常高表达，并与T-ALL的转移密切相关；另有报道，急性淋巴细胞性白血病细胞的转移与基质金属蛋白激酶MMP-2、MMP-9异常高表达有关；并且前列腺癌中CCL25/CCR9可促进MMP-2、9的表达，增强肿瘤细胞的转移能力；而有关CCR9与MMP-2、MMP-9参与T-ALL浸润转移的研究国内外尚未见报道。本研究拟以天然高表达CCR9的T-ALL细胞系MOLT4及临床样本为研究对象，运用RNAi、RT-PCR、Western Blot等技术，试图证实在CCL25/CCR9介导的T-ALL转移过程中，MMP-2和MMP-9 起着关键作用。以期进一步阐明T-ALL转移的分子机制，为治疗T-ALL提供新的线索。

Recently, many studies have shown that chemokines and their receptors play an important role in the process of tumor metastasis. Our previous studies have confirmed that high expression of CCR9 on the lymphocytes of patients with T–lineage acute lymphocytic leukemia (T-ALL) is closely related to tumor metastasis. Other studies have reported that metastasis of acute lymphocytic leukemia cells is related with the high expression of Matrix metalloproteinase 2 (MMP-2) and Matrix metalloproteinase 9 (MMP-9), and that CCL25/CCR9 can promote the expression of MMP-2, 9 and enhance the metastasis of tumor cells in prostate cancer. However there has been no evidence to suggest that CCR9, MMP-2 and MMP-9 are involved in the metastasis of T-ALL. We therefore intend to investigate whether MMP-2 and MMP-9 play key roles in the metastasis of T-ALL in MOLT4 cells and T-ALL clinical samples by employing RNAi, RT-PCR, Western Blot techniques. Our research will shed light on the molecular mechanisms of metastasis of T-ALL thereby providing new clues in the treatment of T-ALL.