转染过程复杂、细胞损害严重以及转染存活率低等问题已成为制约诱导性多能干细胞（iPSCs）技术走向应用的主要瓶颈，而纳米自来水笔（NFP）将蘸水笔纳米印刷术和微流控技术相结合，能够实现精确、微量和连续输送纳米粒子或生物分子，有望克服以上iPSCs中的瓶颈问题。本项目拟在自然基金项目（91123023）的基础上，发展一套基于NFP技术的纳米输送与操作新方法，实现分子传输与单细胞转染，并研究其在干细胞诱导中的基础问题：①温度、湿度、针尖载荷、电场强度与脉冲驻留时间等对介导过程的促进或抑制规律；②力-电耦合作用下诱导因子在微流道、微流道与针尖、针尖与细胞之间的传输与释放机理；③诱导因子导入后细胞的存活率与诱导成功率研究。本项目有望推动NFP技术在iPSCs中的应用，为发展更为可靠的单细胞转染技术提供有益的借鉴。

Induced pluripotent stem cells (iPSCs) has been proven to be promising in personalized drug toxicology testing, pharmaceutical screening, and disease diagnosis. However, the potential has far outpaced researcher's ability to efficiently generate and test iPSC-derived cells with the desired phenotypes. To address such shortcomings, the vision for the proposed project is to develop nano fountain probe (NFP) based nanomanipulating and nanotesting systems to realize molecular transportation and single cell transfection. Due to the advantages of precision positioning, high resolution deposition, and continuously delivery NFP is promising in improving the cell reprogramming rate. In this project, we propose to investigate three fundamental problems in NFP cell transfection: 1. Temperature, humidity, forces, and electrical field effects on increasing or decreasing the molecule transportation process; 2. The transportation and deposition mechanisms of the inducing factors within the microchannel, between the channel and the tip, and between the tip and the cell; 3. The transfection rate after the inducing factors are delivered into the cell.